'Chasselas Cioutat' Progeny

Breeding Goal: One Leaf to Rule Them All

  • 'Chasselas Cioutat' is a Vitis vinifera grapevine with a unique mutation. This mutation leads to a dissected foliar anatomy; its leaves are so dissected that they are technically compound.

  • This trait is desirable for its functional aesthetic impact. It is beautiful to the eyes, and functional as a means to open the grapevine's canopy.

Five Investigative Crosses

  • In 2018, Andrej Svyantek crossed 'Chasselas Cioutat' pollen onto 'Frontenac gris', E.S. 5-8-17, and V. riparia (ND.B27). Approximately 1000 seeds were generated across all the crosses (although, only ~45% germinated in the Spring of 2019).

  • In 2019, Dr. Matthew Clark and the UMN Grape Breeding Team produced two unique crosses to supplement our NDSU-GGEP populations. A pre-existing breeding line (MN1384) resulting from a cross between V. riparia 89 and 'Chasselas Cioutat' was selfed forming additional S1/F2 lines. Simultaneously, it was backcrossed to 'Chasselas Cioutat' to form an information BC1 population.

Three North Dakota State University Grapevine Germplasm Enhancement Project Populations*

*These experimental lines and progeny of them are not currently on the market nor are they available for sale.

E.S. 5-8-17 × 'Chasselas Cioutat'


'Frontenac gris' × 'Chasselas Cioutat'


V. riparia B.27 × 'Chasselas Cioutat'


The Results of S0/F1 Generation

E.S. 5-8-17 × 'Chasselas Cioutat'=

78 seedlings Wild-type (WT): 0 seedlings mutant/ 'Chasselas Cioutat' type (CC)


'Frontenac gris' × 'Chasselas Cioutat'=

123 WT: 0 CC


V. riparia B.27 × 'Chasselas Cioutat':

225 WT: 1 CC


Conclusion of S0/F1: 'Chasselas Cioutat' based, lacinate leaf shape is not dominantly inherited in the three V. spp. crosses conducted.

Speed Breeding, Like a Turtle

We pushed most retained S0/F1 seedlings through juvenility in under a year within greenhouse conditions.

  • With hermpaphroditic, perfect flowered lines, we generated S1/F2 lines via selfing.

  • For pistillate, female flowered lines, we generated back-cross 1 (BC1) lines using 'Chasselas Cioutat' pollen. We have also been intercrossing among S0/F1 lines from different maternal progenitors to reduce inbreeding effect for future seed lines.

  • These lines are being actively germinated and phenotyped, circa ~Dec. 2019/Jan. 2020 to present.

Seeds Produced by Crosses

Seedlings Germinate

Seedlings Grow

Seedlings Segregate

Segregating Generation

S1/F2 generation screening is on-going using visual assessment and landmark morphometric approaches.


Our preliminary research was highlighted among the 2020 Best Student Video Presentation Awards as part of the American Society for Enology and Viticulture.

Observations on Inheritance of Lacinate Leaves in Hybrid Grapevine Populations Derived from ‘Chasselas Cioutat’

Funding and Support

North Central SARE GNC19-288:

  • NCSARE provides direct financial support for phenotyping and genotyping (Genotyping-by-Sequencing [GBS]) via the following grant-


VitisGen2:

  • As a breeding team collaborator in VitisGen2, we obtained rhAmpSeq genotyping of select lines associated with this project.